Enzyme-linked immunosorbent assays (ELISAs) are a common test to quantify antibody or antigen binding activity. For more detail, see our ELISA page.
Read more about our ELISA Assay Development
The Western blot is a standard technique used to quantify the amount of target protein produced by a cell. For more detail, see our Western Blotting page.
Read more about our Western Blot Services
In addition, various techniques can be used to assess the stability of proteins produced. For more detail, see our Stability Analysis page.
Read more about our Stability Analysis Services
Development of Stable Cell Lines
- Cell engineering and development of cancer cell lines stably expressing protein of interest
- Cloning of gene encoding construct, amplification and purification of pDNA expression construct
- Stable transfection and cell selection
- Development and characterization of cell line
- Protein expression validation: ELISA, WB, or qPCR
Read more about our Stable Cell Line Development
Expression Systems Available
- Mammalian. Example system: Chinese Hamster Ovary (CHO) or any other immortalized cell line
- Bacteria. Example system: Escherichia coli
- Yeast. Example system: Saccharomyces cerevisiae
- Other system of the client’s choice
Purification Methods Available
- Affinity Chromatography
- Size Exclusion Chromatography
- Ion-Exchange Chromatography